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3D drift correction for super-resolution imaging with a single laser light
Optics Letters- received 01/24/2024; accepted 04/15/2024; posted 04/16/2024; Doc. ID 519290
- Abstract: Single-molecule localization microscopy (SMLM) enables three-dimensional (3D) super-resolution imaging of nanoscale structures within biological samples. However, prolonged acquisition introduces drift between the sample and the imaging system, resulting in artifacts in the reconstructed super-resolution image. Here, we present a novel 3D drift correction method that utilizes both the reflected and scattered light from the sample. Our method employs the reflected light of a near-infrared (NIR) laser for focus stabilization while synchronously capturing speckle images to estimate lateral drift. This approach combines high-precision active compensation in the axial direction with lateral post-processing compensation, achieving the abilities of the 3D drift correction with a single laser light. Compared to the popular localization events-based cross-correlation method, our approach is much more robust, especially for datasets with sparse localization points.