Abstract
A non-invasive detection method for the status analysis of cell culture is
presented based on digital holography technology. Lensless Fourier transform digital
holography (LFTDH) configuration is developed for living cell imaging without
prestaining. Complex amplitude information is reconstructed by a single inverse fast
Fourier transform, and the phase aberration is corrected through the two-step phase
subtraction method. The image segmentation is then applied to the automatic
evaluation of confluency. Finally, the cervical cancer cell TZMbl is employed for
experimental validation, and the results demonstrate that LFTDH imaging with the
corresponding image post-processing can provide an automatic and non-invasive
approach for monitoring living cell culture.
© 2011 Chinese Optics Letters
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