Abstract
Based on the floating reference theory, a new method for extracting the net
analyte signal (NAS) is proposed. The noise background subspace is spanned by
spectra at the floating radial reference point, and then, the spectra at the
measurement point are projected on the subspace. Thereafter, the glucose
concentrations in intralipid solutions are investigated through Monte Carlo
simulation and experiments, and the partial least squares (PLS) models with and
without NAS analysis are built. The root mean square errors of calibration and
prediction reach to 28.87% and 27.33%, respectively. The results confirm the
existence of information induced by glucose concentration variations as well as the
validity of the floating reference theory.
© 2012 Chinese Optics Letters
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