Abstract
A nondestructive analytical method based on the use of Fourier transform Raman spectroscopy (FT-RS) for identification and quantitative analysis of hydroxyapatite (HAP) and collagen (COL) in bones was developed. A calibration curve based on the Raman spectra of known powder mixtures of HAP-COL was constructed with the use of the Raman bands at 960 cm<sup>-1</sup> for HAP and 2937 cm<sup>-1</sup> for COL. It was found that <i>I</i><sup>960</sup>/<i>I</i><sup>2937</sup> = 0.46/W<sub>c</sub> - 0.36, where I<sup>960</sup> and I<sup>2937</sup> are the relative intensities for the 960 and 2937 cm<sup>-1</sup> Raman bands, respectively, and W<sub>c</sub> represents the weight fraction of collagen. The detection limit for HAP and COL was found to be 2.8 and 0.6 wt %, respectively. The potential of using RS for an <i>in vitro</i> point-by-point analysis ("mapping") of bones was demonstrated successfully on an artificially demineralized ox femur sample.
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