Abstract
A strategy for the infrared spectroscopic analysis of drug metabolites in high-pressure liquid chromatography (HPLC) effluent is presented with a primary focus on delivery of metabolite structure as opposed to development of dedicated or automated instrumentation. This paper stresses the complementary nature of on-line (solution phase) and off-line (condensed phase) HPLC Fourier transform infrared (FT-IR) implementations for structural elucidation of drug metabolites.
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