Abstract
The nondestructive detection of analyte spots on high-performance thin-layer chromatography (HPTLC) plates is demonstrated with the use of near-IR spectrometry (NIRS). The resulting hyphenated technique is called Thin-Layer Chromatography-Near-Infrared Spectrometry (TLC-NIRS). A transmittance geometry is employed, and a set of 18 interference filters is used for wavelength selectivity. Plate-thickness nonuniformity and varying amounts of water vapor adsorption onto the silica-gel TLC plates were found to be the major complications. Variations in silica-gel thickness could be compensated for by obtaining reference spectral scans of the HPTLC plates before performing the chromatography. The effects of water-vapor adsorption were reduced through use of a partial least-squares calibration model. Detection limits near 1 μg were obtained.
PDF Article
More Like This
On-line dynamic detection in the column chromatography separation based on an optical fiber surface plasmon resonance sensor
Jiaming Cao, Xinghua Yang, Pingping Teng, Zhihai Liu, Jun Yang, Jianzhong Zhang, Yu Zhang, Meng Luo, Danheng Gao, Depeng Kong, Siyuan Xia, Enming Zhao, and Libo Yuan
Appl. Opt. 58(21) 5774-5779 (2019)
Cited By
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription