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Feasibility of multimodal multiphoton microscopy to facilitate surgical margin assessment in pancreatic cancer: erratum

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Abstract

After publication of our original paper Appl. Opt. 59, G1 (2020) [CrossRef]  , it came to the authors’ attention that there were missing labels on Fig. 5. The labels are important for our readers to understand the figure.

© 2020 Optical Society of America

In Ref. [1], the original version of Fig. 5 was published without labeling. The corrected version with labeling is shown as follows:

 figure: Fig. 5.

Fig. 5. Comparison between multi-photon microscopy and conventional light microscopy in imaging of cancerous pancreas with a remnant border of normal pancreas. The H&E image was captured with conventional light microscopy image and was viewed at ${10\times}$ magnification. The above image shws normal structures of a lobule consists of acini along with blood vessels, whereas in the cancerous tissue there is a loss of regular cell morphology and architecture. The 2PEF and 3PEF images display similar structures as seen in the H&E image. The SHG clearly shows build up in the boundary region of collagen. The THG signal revealed prominent and irregular nuclei in the tumor region. The combined four signal MPM image provides detailed visualization of the ECM and cell morphology (2PEF = red, 3PEF = cyan, SHG = green, THG = blue). Images on the side provide additional color coded highlight regions at higher magnification. Two small regions near the border (1 and 2) are shown, indicated where the data from Fig. 6 came from.

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REFERENCE

1. T. Pham, B. Banerjee, B. Cromey, S. Mehravar, B. Skovan, H. Chen, and K. Kieu, “Feasibility of multimodal multiphoton microscopy to facilitate surgical margin assessment in pancreatic cancer,” Appl. Opt. 59, G1–G7 (2020). [CrossRef]  

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Figures (1)

Fig. 5.
Fig. 5. Comparison between multi-photon microscopy and conventional light microscopy in imaging of cancerous pancreas with a remnant border of normal pancreas. The H&E image was captured with conventional light microscopy image and was viewed at ${10\times}$ magnification. The above image shws normal structures of a lobule consists of acini along with blood vessels, whereas in the cancerous tissue there is a loss of regular cell morphology and architecture. The 2PEF and 3PEF images display similar structures as seen in the H&E image. The SHG clearly shows build up in the boundary region of collagen. The THG signal revealed prominent and irregular nuclei in the tumor region. The combined four signal MPM image provides detailed visualization of the ECM and cell morphology (2PEF = red, 3PEF = cyan, SHG = green, THG = blue). Images on the side provide additional color coded highlight regions at higher magnification. Two small regions near the border (1 and 2) are shown, indicated where the data from Fig. 6 came from.
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