Abstract
A fluorescence polarization instrument, in which a heterogeneous population of biological cells can be characterized according to the fluidity of their plasma membranes, is described. The analyzed cells are first labeled with a fluorescent dye specific to the lipid bilayer of the cell membrane, after which the fluidity of each cell membrane is determined separately by recording the degree of fluorescence polarization. The measurements are automatically controlled, and the fluorescence signal is detected when a cell is passing in front of a microscope objective. The distribution of the cell population as a function of the fluorescence polarization is computed and can be stored or displayed at will.
© 1977 Optical Society of America
Full Article | PDF ArticleMore Like This
Xuanling Li, Zhaowu Chen, Yinmei Li, Hanci Liang, Haowei Wang, and Ming Li
Opt. Express 29(8) 11976-11986 (2021)
Giada Bianchetti, Marco De Spirito, and Giuseppe Maulucci
Biomed. Opt. Express 11(10) 5728-5744 (2020)
Yuanqing Ma, Aleš Benda, Philip R. Nicovich, and Katharina Gaus
Biomed. Opt. Express 7(4) 1561-1576 (2016)